Friederike Hesse;Eva Maria Trost-Gross;Eva Hoess;Stephanie Mueller
发明人:
Friederike Hesse,Eva Hoess,Stephanie Mueller,Eva Maria Trost-Gross
申请号：
US13577284
公开号：
US20120309679A1
申请日:
2011.02.04
申请国别(地区):
US
年份:
2012
代理人:
摘要：
Herein is reported a method for producing of a polypeptide conjugated to one poly (ethylene glycol) comprising a) providing a nucleic acid encoding an expression construct comprising in 5′ to 3′ direction a nucleic acid encoding a polypeptide, and a nucleic acid encoding a trypsin site of SEQ ID NO: 01, b) expressing the nucleic acid of a) in a cell and recovering the expression construct from the cell and/or the cultivation medium, c) providing a target peptide with an amino acid sequence of SEQ ID NO: 02 covalently conjugated to a poly (ethylene glycol) at the C-terminal lysine residue, d) incubating the expression construct and the target peptide with the trypsin mutant D189K, K60E, N143H, E151H, and e) recovering and thereby producing the polypeptide conjugated to one poly (ethylene glycol) from the incubation mixture.
