HESSE FRIEDERIKE,HOESS EVA,MUELLER STEPHANIE,TROST GROSS EVA MARIA
申请号:
IN1842/MUMNP/2012
公开号:
IN2012MN01842A
申请日:
2012.07.25
申请国别(地区):
IN
年份:
2013
代理人:
摘要:
Herein is reported a method for producing of a polypeptide conjugated to one poly (ethylene glycol) comprising a) providing a nucleic acid encoding an expression construct comprising in 5 to 3 direction a nucleic acid encoding a polypeptide and a nucleic acid encoding a trypsin site of SEQ ID NO: 01 b) expressing the nucleic acid of a) in a cell and recovering the expression construct from the cell and/or the cultivation medium c) providing a target peptide with an amino acid sequence of SEQ ID NO: 02 covalently conjugated to a poly (ethylene glycol) at the C terminal lysine residue d) incubating the expression construct and the target peptide with the trypsin mutant D189K K60E N143H E151H and e) recovering and thereby producing the polypeptide conjugated to one poly (ethylene glycol) from the incubation mixture.