The present invention provides a dipeptidyl with a peptidase IV (DPPIV) inhibitory action, oral intake to high safety bonito soup or bonitos a composition derived from lees. As a bonito or deriveds a bonito DPPIV inhibiting composition derived from the lees. Method of manufacturing comprising the following steps, dipeptidyl peptidase IV inhibiting composition deriveds a bonito lees. 1) dried bonito was extracted with 60 ~100 of hot water, further removes the above supernatant by centrifugation, obtaining a bonitos a meal 2) Its the bonito meal, optimal conditions, selected from the specific group at least it is one of the strains of the protease derived from the enzymatic degradation by the food and industrial applications of the protease, further obtaining a clear solution by centrifugation to be a process to purify the 3) the clear solution and 4) ethanol concentration of 10% , 25%, steps BACKGROUND oF performing elution with 50% and 99.5% stepwise gradient FIG 1【課題】ジペプチジルペプチダーゼIV(DPPIV)阻害作用を有する、経口摂取して安全性の高い鰹だし又は鰹だし粕由来の組成物の提供。【解決手段】鰹だし由来の又は鰹だし粕由来のDPPIV阻害組成物。以下の工程を含む、鰹だし粕由来のジペプチジルペプチダーゼIV阻害組成物の製造方法。1)鰹節を60℃~100℃の熱水で抽出し、更に遠心分離によって上澄を取り除き、鰹だし粕を得る工程;2)前記鰹だし粕を、至適条件下、特定の群より選択される少なくとも1種の菌株由来のプロテアーゼであって食品工業用途のプロテアーゼによって酵素分解し、さらに遠心分離によって清澄液を得る工程;3)前記清澄液を精製する工程;及び4)エタノール濃度10%、25%、50%及び99.5%のステップワイズグラジエントによる溶出を行う工程【選択図】図1
