A microsphere making device includes a substrate, a first micro channel, a second micro channel, a first mix micro channel and a third micro channel, which are located inside the substrate. The first micro channel includes a fore-guiding micro channel and a space inside a thin long tube embedded in the substrate, which are connected together. An end of the thin long tube has a thin tube outlet and the first micro channel is set at a first inlet of the for-guiding micro channel. Two second micro channels respectively have a second inlet, and the second micro channels respectively connect the thin tube outlet of the first micro channel at the left and right sides of the micro channel so that a configuration of three-way confluence is formed. The first mix micro channel connects the confluence where the thin tube outlet and the two second micro channels meet. The first mix micro channel has an outlet. The third micro channel connects one side of the first mix micro channel and has a third inlet.一種微球製造裝置包含:基板及基板內的第一微流道、第二微流道、第一混合微流道及第三微流道。第一微流道包含前後相連接的前引微流道與嵌附在基板內的一長細管的管內空間,長細管尾端具有一細管出口端,第一微流道設置在前引微流道的第一注入口;二第二微流道分別具有第二注入口,且第二微流道分別由第一微流道的左右二側連通第一微流道的細管出口端而形成三方匯合的形態;第一混合微流道連接於細管出口端與二第二微流道匯合處,第一混合微流道具有出口端;第三微流道連通第一混合微流道的一側,其具有一第三注入口。20’‧‧‧微球製造裝置21‧‧‧基板22‧‧‧第一微流道221‧‧‧前引微流道222‧‧‧長細管2221‧‧‧管內微流道空間2222‧‧‧細管出口端2223‧‧‧注入孔223‧‧‧第一注入口23‧‧‧第二微流道231‧‧‧第二注入口24’‧‧‧第一混合微流道241‧‧‧出口端25’‧‧‧第三微流道251‧‧‧第三注入口A,B‧‧‧液滴F,F’,F”‧‧‧流體V1,V2‧‧‧錐狀結構