A method and system for the decontamination of poultry houses between flocks where the building is evacuated of all animals and people, and the poultry house, in particular the bedding, is saturated with high levels of ozone, preferably using a portable and transportable ozone supply and delivery system. A tarp may be used over top of the bedding and the ozone delivered beneath the tarp to concentrate the ozone to the bedding. The system may also be used to decontaminate water lines in the poultry house.
