The present invention provides four purified preparation containing a polynucleic acid molecule encoding porcine Torque teno virus (PTTV) genotypes or subtypes PTTV1 a-VA, PTTV1 b-VA, PTTV2b-VA, and PTTV2c-VA. The present invention also provides infectious DNA clones, biologically functional plasmid or viral vector containing the infectious nucleic acid genome molecule of the same. The present invention further provides live, attenuated, vector-expressed and purified recombinant capsid subunit or killed viral vaccines for protection against PTTV infection. The present invention additionally provides subunit vaccines comprising PTTV specific gene products, especially ORF1 capsid gene product for protection against PTTV infection. Further, the present invention provides methods for diagnosing PTTV infection via polymerase chain reaction (PCR) using specific primer for PTTV1, PTTV2, and individual PTTV1 genotypes. Finally, the present invention provides methods for diagnosing PTTV infection via immunological methods, e.g., enzyme-linked immunoabsorbent assay (ELISA) and Western blot using PTTV specific antigens for detecting serum PTTV specific antibodies.Una vacuna que comprende un portador fisiológicamente aceptable. no tóxico y una cantidad ínmunogénica de una secuencia polipeptídica ORF1 de un virus Torque teno porcino (PTTV) 2c-VA completo, o un fragmento de subtipo inmunogénico especifico del mismo, en donde la proteína ORF1 o fragmento inmunogénico de la proteína ORF1 comprende al menos una región hipervariable (HVR) seleccionada de los aminoácidos 363 a 375, y 388 a 423 de la secuencia SEQ ID NO 16； Un método para diagnosticar infección por PTTV2c-VA； Una vacuna de Torque teno porcino (PTTV) 2c-VA.
