The invention provides novel methods and compositions for transfer of nuclear and/or plastomic genomes, or portions thereof, or cytoplasmic component(s) and/or genetic material, between plant cells. Methods for production of a wounded mixed cell culture, or mixing two or more cell cultures after wounding, and transfer of genetic and/or cytoplasmic component(s), such as transfer of nuclear and/or plastid gene(s) or mutations, edits or alleles, between cells of the mixed culture, are also provided. Wounded mixed cell cultures produced by such methods, and resulting cells and regenerated plants, plant parts, and progeny plants are further provided. Molecular and genetic analyses, and screenable and selection markers, are also provided to confirm transfer and presence of cytoplasmic and/or nuclear component(s) and/or gene(s), mutation(s) or allele(s) in cells and plants produced by these methods.
