SHAOXING INSTITUTE OF TECHNOLOGY,COLLEGE OF ENGINEERING,PEKING UNIVERSITY BIOENGINEERING CENTER
发明人:
HU, GUANG,ZHANG, MING,TU, HUIHUI
申请号：
WO2013CN78102
公开号：
WO2014139240(A1)
申请日:
2013.06.27
申请国别(地区):
世界知识产权组织国际局
年份:
2014
代理人:
摘要：
The present invention relates to the technical field of inspection and measurement. Disclosed is an enzymology detection method for detecting concentration of citrulline and asymmetric dimethylarginine (ADMA) in a reaction system by using an ATP detection method. The basic principle of the reaction is mainly as follows: ADMA is hydrolyzed by using dimethylarginine dimethylaminohydrolase to obtain citrulline; when ADP and Mg2+ exist, the citrulline is coupling-catalyzed by using ornithine carbamoyltransferase and carbamoyl phosphate kinase to finally obtain ornithine, carbon dioxide, ammonia gas, and ATP, wherein the released ATP can be detected by using a reagent. The present invention has the advantages of flexible and quick reaction, high accuracy, and low cost; ADMA or citrulline with the concentration of 0.1μmol/L at lowest can be detected, and the detection result approaches the concentration of ADMA in the normal serum and is far less than the concentration of citrulline in the normal serum, thereby pr
