PROBLEM TO BE SOLVED: To provide a biological material containing scaffold for wound healing.SOLUTION: There is provided: a biological material containing a scaffold crosslinked between any two among collagen, hyaluronic acid and gelatin via ethyl-3-[3-dimethylaminopropyl] carbodiimide (EDC), and comprising collagen, hyaluronic acid and gelatin. There are also provided: a method for preparing the biological material and a method for improving wound healing using the biological material.