The present disclosure relates to T cells transduced with a viral vector at a volumetric concentration for immunotherapy and methods thereof. In another aspect, the present disclosure relates to the assessment of optimal lentiviral vector concentrations for transducing T cells. The present disclosure further provides for T cell populations produced by methods described herein.本公開涉及用一體積濃度的病毒載體轉導的用於免疫療法的 T 細胞及其方法。另一方面,本公開涉及用於轉導 T 細胞的最佳慢病毒載體濃度的評估。本公開進一步提出了透過本文所述方法產生的 T 細胞群。