Advanced Cell Technology and Engineering Ltd.;Kyushu University National University Corporation
发明人:
申请号：
EP18747960.5
公开号：
EP3578641A1
申请日:
2018.02.01
申请国别(地区):
EP
年份:
2019
代理人:
摘要：
[Problem] The present invention addresses the problem of providing a method for efficiently preparing dental pulp stem cells from dental pulp cells collected from dental pulp tissue. [Solution Means] The method for preparing dental pulp stem cells from dental pulp cells collected from dental pulp tissue according to the present invention includes: (a) a step for preculturing dental pulp cells using a culture medium that includes serum at 7-13% (v/v), the dental pulp cells being bonded to a culture dish； (b) a step for culturing the dental pulp cells bonded to the culture dish by step (a) at least for one day using a culture medium that includes serum at 7-13% (v/v)； and (c) a step for culturing the dental pulp cells in a low-serum condition after step (b).
