KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION;ADVANCED CELL TECHNOLOGY AND ENGINEERING LTD.
发明人:
YAMAZA Takayoshi,SONODA Soichiro,HAMAZONO Toshiro
申请号：
SG11201907134V
公开号：
SG11201907134VA
申请日:
2018.02.01
申请国别(地区):
SG
年份:
2019
代理人:
摘要：
IProblem] The present invention addresses the problem of providing a method for efficiently preparing dental pulp stem cells from dentaf pulp cel-ls col-.-ected from dental pulp tissue. ISolution Means] The method for preparing dental pufp stem cell-s from dental pufp cefls collected from dental pulp tissue according to the present invention includes: (a) a step for preculturing dentaf pufp cefl-s using a culture medium that i-ncludes serum at '7 -I3Z (v /v) , the dentaf pufp cel-ls being bonded to a culture dish； (b) a step for cuJ-turing the dental- pulp cel-fs bonded to the cul-ture dish by step (a) at least for one day using a culture medium that incl-udes serum at 1 -I3z (v /v) ； and (c ) a step f or culturing the dental pulp cel-1s in a low-serum condition after step (b) . 61
