Auerbach Wojtek,Frendewey David,Droguett Gustavo,Gagliardi Anthony,Kuno Junko,Valenzuela David M.
申请号：
US201514731914
公开号：
US10106820(B2)
申请日:
2015.06.05
申请国别(地区):
美国
年份:
2018
代理人:
Choi Yongjin`Alston & Bird LLP
摘要：
Methods and compositions are provided for modifying one or more target loci in a cell. Such methods comprise providing a cell comprising a first polynucleotide encoding a first selection marker operably linked to a first promoter active in the cell, wherein the first polynucleotide further comprises a first recognition site for a first nuclease agent. A first nuclease agent is introduced into a cell, wherein the first nuclease agent induces a nick or double-strand break at the first recognition site. Further introduced into the cell is a first targeting vector comprising a first insert polynucleotide flanked by a first and a second homology arm that correspond to a first and a second target site located in sufficient proximity to the first recognition site. At least one cell is then identified comprising in its genome the first insert polynucleotide integrated at the target locus.
