The present invention provides a new supply source of phospholipase A1, which is useful for phospholipid modification and lipid biochemical research, and offers a method capable of efficiently manufacturing a large amount of high DHA content phospholipids and lysophospholipids. A novel microorganism HFKl-0020 (FERM AP-20545) of the genus Pseudomonas which produces enzymes with phospholipase A1 activity can be used as a new supply source of enzymes with the phospholipase A1 activity. A lipid-modifying agent containing an effective dose of enzymes with the phospholipase A1 activity produced by those novel microorganisms allows the efficient mass-production of lysophospholipids.
