The present invention is directed to microbial oils that are enriched in their content of polyunsaturated fatty acids, their esters, their acid salts, alcohols formed therefrom, and/or aldehydes formed therefrom. The present invention is further directed to compositions containing the enriched microbial oils and methods of making and using the enriched microbial oils. The present invention is also directed to isolated microorganisms as well as strains and mutants thereof, biomasses, microbial oils, compositions, and cultures; methods of producing the microbial oils, biomasses, and mutants; and methods of using the isolated microorganisms, biomasses, and microbial oils.本発明は、多価不飽和脂肪酸、そのエステル、その酸性塩、それから形成されるアルコール、及び/又はそれから形成されるアルデヒドの含量が高濃度化された微生物油に関する。本発明はさらに、高濃度化微生物油を含有する組成物及び高濃度化微生物油の作製及び使用方法に関する。本発明はまた、単離微生物並びにその株及び突然変異体、バイオマス、微生物油、組成物、及び培養物;微生物油、バイオマス、及び突然変異体の生成方法;及び単離微生物、バイオマス、及び微生物油の使用方法にも関する。