The present invention addresses the problem of providing an artificial skin preparation which requires no suturing or the like when treating a wound area with a dried collagen vitrigel membrane material as an artificial skin, and also is less likely to cause contamination or the like with an exudate, and is free from the risk of secondary damage by replacement thereof. The artificial skin preparation for solving the problem includes at least an adhesive film, an adhesion-preventing sheet, and a dried collagen vitrigel membrane material in this order, and is characterized in that the adhesion-preventing sheet has a sufficient size for preventing the adhesion of the collagen vitrigel membrane to an adhesive layer of the adhesive film and is attached to the adhesive layer of the adhesive film at a position corresponding to the position of the dried collagen vitrigel membrane material, and the dried collagen vitrigel membrane material is made easily detachable from the adhesion-preventing sheet when the adhesive film is peeled off while fixing and holding the dried collagen vitrigel membrane material on the surface of the adhesion-preventing sheet during attachment.