GRIESHOBER, WILLIAM E.,JONES, JAMES S.,KOGAN, SEMYON,ILYIN, ILYA,ENUKASHVILY, NATELLA I.,FILKINA, YANA A.,SHUMEEV, ALEXANDER N.,KOLCHANOV, STANISLAV A.
申请号：
CA2824948
公开号：
CA2824948A1
申请日:
2012.02.03
申请国别(地区):
CA
年份:
2012
代理人:
摘要：
Provided is a method for reducing apoptosis in nucleated cells. The method entails holding nucleated cells in a container and adding a gas containing xenon to the container so that the pressure inside the container reaches between 0.5 to 4.0 Atm above ambient pressure； holding the container at between 0.5 to 4.0 Atm above ambient pressure for a period of time during which the temperature in the container is between 22°C and 37°C； lowering the temperature in the container to between 0.1 °C and 10°C while maintaining the pressure of 0.5 to 4.0 Atm above ambient pressure and holding the container for a period of time； and reducing the pressure in the container to ambient pressure and increasing the temperature to 22°C - 37°C. By performing these steps, the cells undergo less apoptosis than a reference.
