The present invention relates to a PCR heating block having heaters repeatedly arranged thereon is capable of preventing the radial thermal distribution generated from the individual heaters and the non-uniform heat superposition between the adjacent heaters to improve the PCR yield and further capable of requiring no separate temperature controlling means to achieve the miniaturization and integration of a device. According to the present invention, furthermore, a PCR device is capable of amplifying a plurality of nucleic acid samples at the same time and rapidly by using a PCR heating block on which heater units are repeatedly arranged and a plate-shaped PCR reaction unit and also capable of measuring successively generated optical signals or electrochemical signals to in real time check the nucleic acid amplification.
