PURPOSE: A method for preparing physiologically active fucoidan using a tangleweed root extract is provided to prevent the loss of active ingredients in tangleweed roots through low temperature extraction and to improve extraction yield and the purity of a fucoidan marker.CONSTITUTION: A method for preparing physiologically active fucoidan using a tangleweed root extract comprises the steps of: removing foreign materials from dried tangleweed roots (S100) crushing the dried tangleweed roots (S200) putting the dried tangleweed roots into a roasting machine and spraying with alcohol rotating at low speed closing the roasting machine when the fragrance of the tangleweed roots is vaporized adding flavor to the tangleweed roots and plasticizing (S300) washing the plasticized tangleweed roots (S400) adding distilled water to the tangleweed roots, adding lyase, and extracting the tangleweed roots at 35-50 °C (S500) adding distilled water to the tangleweed roots and extracting in a vacuum condition at 35-50 °C (S600) putting the extract into a fermentor and culturing one or more kinds of strains in the extract for 10-30 hours (S700) and isolating and purifying the fermented culture liquid and distilling (S800).COPYRIGHT KIPO 2013[Reference numerals] (AA) Start (BB) First process (CC) Second process (DD) Third process (EE) End (S100) Wash (S200) Crush granules (S300) Thermal plasticizing (S400) Wash with distilled water (S500) Enzymatic lysis/extraction at low temperature (S600) Extract under a vacuum condition at low temperature (S700) Fermenting with microorganism (S800) Isolation/purification/distillation/concentration본 발명의 다시마 뿌리 추출물을 이용한 생리활성 후코이단 제조방법은 건조된 다시마뿌리에서 이물질을 제거하여 일정한 크기로 그래뉼 파쇄하는 단계, 그래뉼 파쇄된 다시마뿌리를 덖음기에 투입한 후 알콜을 분사하고 덖음기 내부 온도가 70℃~80℃인 상태에서 저속 회전시키고, 다시마뿌리 고유의 향이 기화되기 시작하면 덖음기를 밀폐하여 덖음기의 내부온도가 100~130℃를 넘지않도록 설정하여 고속 회전시켜 다시마뿌리에 향덧입히고 열 소성하는 단계, 및 열 소성된 다시마뿌리를 증류수로 세척하는 단계를 포함하는 제1공정 다시마뿌리에 증류수를 가한 후 분해효소를 첨가한 후 35℃~50℃의 온도에서 저온추출하는 단계, 및 저온추출을 마친 다시
