The invention refers to a needle for follicular aspiration, comprising a base with a through-hole, inside of which a needle cannula is coupled, the needle cannula having a front aspiration end which protrudes from a front end of the base, and a rear end which freely protrudes from the rear end of the base, the through-hole of the base having a frustro-conical segment tapering from the rear end towards the front end of the base, forming a gap between the inner surface of the base and the outer surface of the needle cannula for coupling a biological sample collecting line.
