The invention relates to a follicular aspiration needle comprising a base (2) with a through-hole, inside of which a needle cannula (1) is coupled, the needle cannula (1) having a front aspiration end (11) which protrudes from a front end of the base (2) and a rear end (9) which freely protrudes from the rear end of the base (2), the through-hole in the base (2) has a frusto-conical segment (22) tapering from the rear end to the front end of the base (2), forming a gap (6) between the inner surface of the base (2) and the outer surface of the needle cannula (1) for coupling a biological sample collecting line (7).
