Method for suppressing intracellularly activated RAS using antibody having cytoplasmic penetrating ability in complete immunoglobulin form and use thereof
The present invention relates to a method for suppressing intracellularly activated RAS using an antibody having cytoplasmic penetrating ability in the form of a complete immunoglobulin (immunoglobulin). In addition, the present invention is directed to the binding of an intact immunoglobulin form of an antibody to the cytoplasm of a living cell through endocytosis and endosome escape and activating cytoplasmic activation. The present invention relates to a heavy chain variable region (VH) that induces and an antibody comprising the same. The present invention also relates to a method for suppressing the growth of cancer or tumor cells using the antibody and a method for treating cancer or tumor. The present invention also relates to a method for screening a heavy chain variable region that specifically binds to cytoplasmic RAS. The present invention also relates to a bioactive molecule selected from the group consisting of peptides, proteins, small molecule drugs, nanoparticles and liposomes fused to the antibody. The present invention also provides a composition for preventing, treating or diagnosing cancer comprising the antibody or a bioactive molecule selected from the group consisting of peptides, proteins, small molecule drugs, nanoparticles and liposomes fused thereto. About. The present invention also relates to the polynucleotide encoding the light chain variable region and the antibody. The method of suppressing intracellularly activated RAS using an antibody having cytoplasmic penetrating ability in the form of a complete immunoglobulin provided in the present invention, wherein the antibody penetrates inside living cells. Activated (GTP-conjugated) RAS located in the cytoplasm is achieved by an antibody capable of specific recognition, thereby activating (GTP) located in the inner cytoplasm of living cells. RAS can be targeted and its activity can be inhibited. Further, the light chain variable region of the antibody provided in the present invention or
