The present invention concerns a method for eliminating microorganisms present in and/or at the surface of a material to be decontaminated comprising a step of irradiating said material to be decontaminated with radiation consisting of at least two light beams α1 and α2 directed onto said material, the two light beams α1 and α2 respectively having a wavelength λ1 and λ2 of between 380 and 420 nm.