Provided are methods, systems, and kits for cell processing, e.g., for therapeutic use, such as for adoptive cell therapy. The provided methods include transduction methods, in which cells and virus are incubated under conditions that result in transduction of the cells with a viral vector. The incubation in some embodiments is carried out in an internal cavity of a generally rigid centrifugal chamber, such as a cylindrical chamber made of hard plastic, the cavity of which may have a variable volume. The methods include other processing steps, including those carried out in such a chamber, including washing, selection, isolation, culture, and formulation. In particular, the disclosure relates to method providing advantages over available processing methods, such as available methods for large-scale processing. Such advantages include, for example, reduced cost, streamlining, increased efficacy, increased safety, and increased reproducibility among different subjects and conditions.本發明提供用於細胞處理(例如用於治療用途，諸如用於授受細胞療法)之方法、系統及套組。所提供之方法包括轉導方法，其中細胞及病毒係在會導致細胞經病毒載體轉導之條件下培育。在一些實施例中，該培育在一般固定的離心室之內部空穴進行，諸如由硬塑膠製成的圓柱形離心室，該空穴具有可變體積。該等方法包括其他處理步驟，包括在此離心室中進行之步驟，包括洗滌、選擇、分離、培養及調配。詳言之，本發明係關於提供優於可用處理方法(諸如可用於大規模處理之方法)之優勢的方法。此類優勢包括例如降低成本、流線化、增加功效、增加安全性及增加在不同個體及條件中的再現性。
