A physiological index detection system includes an electronic device and a mouse communicating with the electronic device. The mouse includes a shell and a physiological index sensing unit attached on the shell. The electronic device includes a display screen. The physiological index sensing unit senses physiological characteristics of a living being who touches the mouse and generates an analog physiological index signal. The physiological index detection system processes the analog physiological index signal to obtain a result in health terms and displays the result on the display screen. The disclosure also provides a mouse and a physiological index detecting method.一種生理指標檢測系統包括生理指標感測單元。該生理指標感測單元用於感測一與之接觸的生命體的生理指標並產生類比生理指標信號,該生理指標檢測單元設置於一滑鼠的外殼上,該生理指標檢測系統還包括一與滑鼠通信的電子裝置,該電子裝置包括一顯示幕,該生理指標檢測系統將類比生理指標信號進行處理並將處理結果顯示於顯示幕上。本發明還提供了一種滑鼠及生理指標檢測方法。10‧‧‧生理指標檢測系統22‧‧‧顯示幕34‧‧‧生理指標感測單元40‧‧‧信號轉換單元50‧‧‧顯示控制單元60‧‧‧資料庫70‧‧‧分析單元80‧‧‧建議單元