A physiological index detection system includes a computer and a mouse, the mouse includes a shell and a physiological index sensing unit and communicates with the computer. The computer includes a display screen and a display control unit. The physiological index sensing unit senses physiological characteristics of a living being who touches the mouse and generates an analog physiological index signal. The physiological index detection system processes the analog physiological index signal to obtain a digital equivalent result in health terms. The display control unit displays the result on the display screen and advice concerning an adverse finding. The disclosure also provides a mouse and a physiological index detecting method.
