A concentration device (10) includes a sample collector (3) and a main chamber (1). In some embodiments, the concentration device includes a solution chamber (4) that holds a concentrating solution that is released from the solution chamber (4) and mixes with the sample obtained by the sample collector (3). In some embodiments, the concentration device includes a microfluidic channel through which sample can move, and a pressure pump may be used to move the sample through the microfluidic channel. The concentration device can be used in performing a method of obtaining a Raman spectrum characteristic of a microbe. The concentration device can be used in performing a method of detecting and identifying a microbe in a sample.