DEMAREST, STEPHEN,GLASER, SCOTT,MILLER, BRIAN ROBERT,WU, XIUFENG,SNYDER, WILLIAM B
申请号：
NZ59125207
公开号：
NZ591252A
申请日:
2007.03.19
申请国别(地区):
NZ
年份:
2012
代理人:
摘要：
Disclosed is a method for designing an antibody or antigen binding fragment thereof, wherein said method comprises: (a) providing a curated reference set of amino acid sequences comprising a sequence corresponding to an immunoglobulin-type fold ("Ig fold") of a protein belonging to the immunoglobulin superfamily of proteins (b) aligning the amino acid sequences of the reference set to generate an aligned set (c) determining covariation between two or more residue positions in the aligned set to generate a covariation dataset (d) identifying non-covarying amino acids in said candidate antibody or antigen binding fragment thereof, wherein corresponding amino acids in the sequences of the reference set are covarying amino acids and (e) substituting non-covarying amino acids in the candidate sequence with corresponding covarying amino acids in the reference set, thereby improving a biophysical property of the candidate sequence.
