The invention relates to a flavonoid metabolite having ability of inhibiting melanogenesis and the uses thereof. The flavonoid metabolite is manufactured by culturing a recombinant yeast comprising a fusion gene of CYP57B3 and CPR in a medium containing a flavonoid for a period of time to conduct a biotransformation. Accordingly, an effective does of the flavonoid metabolite can be applied to a skin for inhibiting tyrosinase activity, so as to inhibit melanogenesis.本發明係有關於一種具抑制黑色素生成能力之類黃酮代謝產物及其用途,其係將一具有CYP57B3與CPR融合基因的重組酵母菌,培養於含有類黃酮的培養基內一段時間,使類黃酮進行生物轉換作用(biotransformation)以製得類黃酮代謝產物;藉此,可施予一有效劑量之類黃酮代謝產物於皮膚,以抑制酪胺酸酶活性,達到抑制黑色素生成之用途。
