A method for determining the zygosity of a canola plant comprising a fad-2 gene, said method comprising: obtaining a sample of genomic DNA from said canola plant； producing a contact sample by contacting said genomic DNA sample with a first primer and a second primer, wherein said first primer consists of SEQ ID NO: 2 that binds a region of said fad-2 gene upstream of a location of a single nucleotide polymorphism of interest, said second primer consists of SEQ ID NO: 3 that binds a region of said fad-2 gene downstream of the single nucleotide polymorphism of interest, wherein said first primer and said second primer produce an amplicon when subjected to polymerase chain reaction (PCR) conditions； subjecting said contact sample to PCR conditions, where said amplicon is produced； allowing each of a first fluorescent probe, the sequence of which consists of SEQ ID NO: 5, and a second fluorescent probe, the sequence of which consists of SEQ ID NO: 4, to hybridize with the amplicon for a period of time and at a temperature between 50-70 degrees Celsius, said first fluorescent probe hybridizing with said amplicon when said single nucleotide polymorphism of interest is not present in said amplicon, said second fluorescent probe preferably hybridizing with said amplicon when said single nucleotide polymorphism of interest is present in said amplicon； increase said temperature after the period of time specified in the allow step； capturing said fluorescence produced by each of said first and second probes during the augmentation step； and determining the zygosity of said canola plant, comprising the step of determining a comparison of the fluorescence produced by each of the first and second probes, wherein the fluorescence of the first and second probes predominantly reflects the fluorescence produced in a sample of SNP homozygous positive control indicating the presence of such a single nucleotide polymorphism of interest, reflecting the fluorescence of the first and seco
