A method for determining the zygosity of a canola plant comprising a fad-3c gene, said method comprising: obtaining a genomic DNA sample from said canola plant； producing a contact sample by contacting said genomic DNA sample with a first primer and a second primer, wherein said first primer consists of SEQ ID NO: 2, which binds to a region of said fad-3c gene upstream of a location of a single nucleotide polymorphism of interest, where said second primer consists of SEQ ID NO: 3, which binds to a region of said fad-3c gene downstream of the single nucleotide polymorphism of interest, in the that said first primer and said second primer produce an amplicon when subjected to the conditions of the polymerase chain reaction (PCR)； subjecting said contact sample to PCR conditions, in which said amplicon is produced； allowing each of a first fluorescent probe, the sequence of which consists of SEQ ID NO: 5, and a second fluorescent probe, the sequence of which consists of SEQ ID NO: 4, to hybridize with the amplicon over a period of time and at a temperature between 50-70 degrees Celsius, said first fluorescent probe preferably hybridizing with said amplicon when said single nucleotide polymorphism of interest is not present in said amplicon, said second fluorescent probe preferably hybridizing with said amplicon when said polymorphism is present in said amplicon. a single nucleotide of interest； increasing said temperature after the period of time specified in the permitting stage； capturing said fluorescence produced by each of said first and second probes during the magnification step； and determining the zygosity of said canola plant, the determination step comprising a comparison of the fluorescence produced by each of the first and second probes, where the fluorescence of the first and second probes predominantly reflects the fluorescence produced in a control sample positive to the homozygous SNP, which indicates the presence of said single nucleotide polymorphism
