SPATZ MARTÍN DIEGO LINUS,GOLDBAUM FERNANDO ALBERTO,ZYLBERMAN VANESA,CRAIG PATRICIO OLIVER,GHERSI GISELLE,PALERMO MARINA SANDRA,MEJIAS MARIA PILAR,BENTANCOR LETICIA VERONICA
申请号：
IN3579/MUMNP/2015
公开号：
IN2015MN03579A
申请日:
2015.12.16
申请国别(地区):
IN
年份:
2016
代理人:
摘要：
Escherichia coliinter aliaThe present invention relates to chimeric polypeptides useful as immunogens for inducing protective immune responses and neutralizing antibodies against Shiga toxin (Stx) in mammals. More specifically the present invention relates to chimeric polypeptides comprising a monomer of the homopentameric B subunit of the Shiga 2 toxin fused to the N terminus of a monomer of Brucella lumazine synthase and to oligomeric protein complexes formed from said chimeric polypeptides. The invention further relates to polynucleotides and vectors encoding said chimeric polypeptides to transgenic cells comprising said polynucleotides and vectors and to pharmaceutical compositions such as a vaccine comprising said chimeric polypeptides and chimeric polynucleotides. Also within the scope of the invention are antibodies which bind to the chimeric polypeptides of the invention a method for obtaining antibodies which bind specifically to the subunit B of the Shiga 2 toxin and methods of lowering the bacterial load of enterohemorrhagic in mammals which can be used for prevention of hemolytic ureic syndrome (HUS).
