The present invention provides a novel human liver regeneration related protein-hLRTM4 protein and polynucleotide for coding hLRTM4 protein. It also discloses the preparation method and application of hLRTM4 protein and its polynucteotide. The hLRTM4 protein can be used for curing injury of liver, and its agonist (for example antisense nucleic acid and antibody) can be used for curing liver cancer. It also provides its correspondent medicine composition.