Generally, the present invention relates to the field of antibodies. In particular, it relates to multispecific antibodies, e.g. bispecific antibodies, which are modified such that the desired chain pairing takes place and/or can be selected for. Specifically, this is achieved by using different dimerization domains for light chain pairing. The invention further relates to nucleic acids encoding for these antibodies, expression vectors comprising these nucleic acids, cells expressing them, and further to pharmaceutical compositions comprising the antibodies. The invention also relates to methods of isolating the antibodies.概括而言，本發明涉及抗體領域。特別地，其關於多特異性抗體，例如雙特異性抗體，其被修飾使得所需的鏈配對發生和/或可以被選擇。具體地，這藉由使用不同的用於輕鏈配對的二聚化結構域達成。本發明進一步關於編碼這些抗體的核酸，包含這些核酸的表現載體，表現它們的細胞，並進一步關於包含所述抗體的醫藥組成物。本發明還關於分離所述抗體的方法。
