The Board of Trustees of the Leland Stanford Junior University
发明人:
Peltz Gary,Xu Dan
申请号：
US201414917558
公开号：
US10004767(B2)
申请日:
2014.09.17
申请国别(地区):
美国
年份:
2018
代理人:
Gurley Kyle A.`Bozicevic, Field & Francis LLP
摘要：
Methods are provided for producing a population of hepatocyte-like cells (iHeps) from a population of adipocyte-derived stem cells (ASCs). Aspects of the methods include placing a population of ASCs into a three dimensional culture (e.g., hanging drop suspension culture, high density culture, spinner flask culture, microcarrier culture, etc.), and contacting the cells with a first and second culture medium. Also provided are methods of treating an individual, which include producing a population of iHeps from a population of ASCs, and administering an effective number of iHeps into the individual. Kits for practicing the methods are also described herein.
