VECTEUR D'EXPRESSION T7 D'ESCHERICHIA COLI, VECTEURS POUR LA CO-EXPRESSION ET LA CO-PURIFICATION DE POLYPEPTIDES RECOMBINANTS DANS/AVEC DES PROTÉINES PORTEUSES, UTILISATION DE VECTEURS D'EXPRESSION POUR L'OBTENTION DE COMPLEXES AVEC DE MULTIPLES ANTIGÈNES ET IMMUNOMODULATEURS
The present invention relates to a vector for the expression of recombinant proteins, antigens, pathogen-like particles and immunogenic complexes, said vector (pMRKA vector) being produced by modifying the plasmids containing the gene sequence of the T7 promoter of E. coli, this modification being mainly characterized by the substitution of the ampicillin-resistance gene by the kanamycin-resistance gene, and by the insertion of the par sequence (partition sequence which determines the efficient segregation of the plasmids in daughter cells during cell division). Also provided are expression vectors based on the pMRKA plasmid, which additionally comprise at least one of the gene sequences of the exosome of P. abyssi, which vectors are designated pMRKA-EXO, pMRKA-RING and pSUMAC. The invention also provides the vectors additionally comprising gene sequences with immunomodulatory or immunoregulatory activity, preferably the pMRKA-Z-Z-EXO and pMRKA-Z-Z-RING vectors. Other aspects of the invention include the method for producing said expression vectors and the use of the obtained vectors.
