A seedling tray removing and unloading device is suitable for picking up a seedling tray on a conveyor. The seedling tray removing and unloading device comprises a moving machine table, a rotating arm, a lifting arm and a jaw assembly. The mobile machine is arranged on the conveyor, and the mobile machine can move along the extending direction of the conveyor. The rotating arm is rotatably disposed on the moving machine. The lifting arm is disposed on the rotating arm and is movably close to or away from the conveyor. The jaw assembly is disposed on the lifting arm for clamping the seedling tray.一種秧苗盤取卸裝置,適於取卸一輸送機上的一秧苗盤。秧苗盤取卸裝置包含一移動機台、一旋轉臂、一升降臂及一夾爪組件。移動機台用以設置於輸送機上,且移動機台可沿輸送機的延伸方向移動。旋轉臂可旋轉地設置於移動機台。升降臂設置於旋轉臂並可移動地靠近或遠離輸送機。夾爪組件設置於升降臂,用以夾放秧苗盤。