A chayote fruit cell fluid, an extraction method of the chayote fruit cell fluid, a skincare and wrinkle-smoothing composition including the chayote fruit cell fluid and the use of the chayote fruit cell fluid are disclosed. The extraction method of the chayote fruit cell fluid includes: squeezing a chayote fruit to obtain chayote fruit juice； first filtering the chayote fruit juice； setting the filtered chayote fruit juice at room temperature for a period； and second filtering the resultant chayote fruit juice to obtain the chayote fruit cell fluid. The method extracts the chayote fruit cell fluid with better skincare and wrinkle-smoothing effects from fresh chayote fruit using a lower temperature process to make a maximum unbroken natural activity of the chayote fruit.本發明提供一種佛手瓜果實細胞原液、其萃取方法、包含其的護膚除皺組合物及其用途。佛手瓜果實細胞原液的萃取方法包含以下步驟：榨取佛手瓜，以取得佛手瓜汁液；對該佛手瓜汁液進行第一次過濾；將經過濾的所述佛手瓜汁液於室溫下靜置沉澱一時間；以及對所述佛手瓜汁液進行第二次過濾以獲得佛手瓜果實細胞原液。此方法以低溫及室溫制程，從新鮮佛手瓜中萃取出具有更佳的護膚除皺效果的佛手瓜果實細胞原液，以求最大限度地使佛手瓜的天然活性不被破壞。
