СПОСОБ ПОЛУЧЕНИЯ КОНЦЕНТРАТА КУЛЬТУРЫ КЛЕТОК БРУЦЕЛЛ ИЗ ШТАММА BRUCELLA ABORTUS 19 ДЛЯ ПРИГОТОВЛЕНИЯ БРУЦЕЛЛЕЗНЫХ АНТИГЕНОВ, БРУЦЕЛЛЕЗНЫЕ АНТИГЕНЫ (ТРИ ВАРИАНТА), СПОСОБ ИЗГОТОВЛЕНИЯ БРУЦЕЛЛЕЗНОЙ ДИАГНОСТИЧЕСКОЙ СЫВОРОТКИ И ТЕСТ-СИСТЕМЫ ДЛЯ ДИАГНОСТИКИ БРУЦЕЛЛЕЗА ЖИВОТНЫХ (ТРИ ВАРИАНТА)
ФЕДЕРАЛЬНОЕ КАЗЕННОЕ ПРЕДПРИЯТИЕ "ЩЕЛКОВСКИЙ БИОКОМБИНАТ" (RU)
发明人:
Тройнин Анатолий Серафимович (RU),Ельников Василий Викторович (RU),Крюкова Елена Николаевна (RU),Сурнев Дмитрий Сергеевич (RU),Мельник Николай Васильевич (RU),Голдина Вера Федоровна (RU),Литенкова Ири
申请号：
RU2014139329
公开号：
RU2014139329A
申请日:
2014.09.30
申请国别(地区):
RU
年份:
2016
代理人:
摘要：
1. A method of producing a culture concentrate cells from a strain of Brucella Brucella abortus Brucella 19 for the preparation of the antigens are used in diagnostic test kits, comprising preparing inoculum of bacterial cells and their culturing conditions in the interior in a liquid culture medium by controlling the concentration level of dissolved oxygen in the culture medium throughout the entire cultivation process, inactivation by heating grown bacterial cells, followed by their concentration, the process for ultivirovaniya bacterial cells is carried out at a temperature of 36-38 ° C, during the culture in step accumulation of bacterial cell concentration of 1.5-2.5 billion MK / cc to a concentration of 10 billion MK / smvyderzhivayut concentration of dissolved oxygen in the culture medium in the range of 20-25 mg / liter, and more than 10 billion MK / cc to achieve bacterial cells provide the stationary growth phase the dissolved oxygen concentration in the culture medium in the range of 40-45 mg / l and the concentration of the grown bacterial cells osusches vlyayut by precipitation flocculant, which is used as the sodium salt of carboxymethyl cellulose (CMC), which was added to the culture medium in an amount of 0.1-015 g dry substance per 1 liter of the culture medium, after which the supernatant was decanted to obtain a concentrate inactivated bacterial cells for the subsequent preparation of Brucella antigen in RA, RSK, RDSK, antigen BPO KR antigen with molokom.2. The method of claim. 1, characterized in that the deposition is carried out for 24-48 hours at a temperature of from 8 ° C to 12 ° C.3. The method of claim. 1, characterized in that the molecules1. Способ получения концентрата культуры клеток бруцелл из штамма Brucella abortus 19 для приготовления бруцеллезных антигенов, используемых в диагностических тест-системах, включающий получение посевного материала бактериальных клеток и их культивирование в глубинных условиях на жидкой питательной сред
