A honeybee behavior monitoring system, including a honeybee behavior monitoring device positioned in a beehive for counting and recording the in-and-out activity of honeybees near a beehive, wherein the honeybee behavior monitoring device includes a first sensing unit and a second sensing unit for generating sensing signals； a counting unit for recording and determining whether honeybees are entering or departing from the beehive； a transmission unit for transmitting the in-and-out activity count to an external device, wherein each honeybee behavior monitoring device transfers data therebetween via a wireless sensing network, and finally data is transmitted to a rear-end server for storage and subsequent analysis of honeybee behaviors according to the status of counting and recording in each honeybee behavior monitoring device and ambient beehive environmental data or meteorological data, thereby providing accurate in-and-out activity counts to facilitate honeybee behavior studies.
