University of Vienna;The Regents of the University of California;Charpentier, Emmanuelle
发明人:
Charpentier, Emmanuelle,Jinek, Martin,Doudna Cate, James Harrison,Lim, Wendell,Qi, Lei,Chylinski, Krzysztof,Doudna, Jennifer A.,Chyli
申请号：
HRP20180794
公开号：
HRP20180794T1
申请日:
2018.05.21
申请国别(地区):
HR
年份:
2018
代理人:
摘要：
The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9； and Cas9 transgenic non-human multicellular organisms.
