Philippe Looten,Samuel Patinier,Eric Francais,Michel Perrut,Aurore Satre-Buisson
申请号：
US14420136
公开号：
US09315434B2
申请日:
2013.08.07
申请国别(地区):
US
年份:
2016
代理人:
摘要：
The invention relates to a method for preparing a composition rich in lutein produced by microalgae, particularly microalgae belonging to the Chlorella genus, more specifically produced by Chlorella vulgaris, characterized in that said method includes the sequence of the following steps: 1) preparing a cell lysate from the microalgae biomass； 2) treating the lysed microalgae biomass with a polar solvent, preferably ethyl acetate, in order to obtain an oleoresin containing the lutein and the lipids from the initial biomass； 3) extracting the oleoresin obtained in step 2) by means of a non-polar solvent, here a fluid at supercritical pressure, in order to obtain a fraction rich in non-polar lipids, mainly consisting of triglycerides, and an insoluble fraction rich in lutein； and 4) recovering the thus lutein-enriched fraction.
