A puncture apparatus is disclosed, which includes puncture means which has a puncture needle for puncturing a biological tissue located in a vicinity of a mid-urethra and biological tissue pushing-in means which has an elongated member to be inserted into one of a urethral lumen and a vaginal cavity, the elongated member being inserted into the one of the urethral lumen and the vaginal cavity and being pressed in the insertion direction thereof, whereby the biological tissue is pushed in the same direction as the pressing direction for the elongated member. The puncture apparatus is used by puncturing the biological tissue with the puncture needle in a state where the biological tissue has been pushed in by the biological tissue pushing-in means.
