An insertion tool is disclosed, which includes an elongate vaginal insertion portion configured to be inserted into a vagina an elongate urethral insertion portion configured to be inserted into a urethra and a supporting portion configured to support the vaginal insertion portion and the urethral insertion portion thereon. In a mounted state of the insertion tool in which the vaginal insertion portion is inserted in the vagina and the urethral insertion portion is inserted in the urethra, a puncture needle can puncture a vaginal wall from an outside of a body through a space defined by the vaginal insertion portion, the urethral insertion portion and the supporting portion.