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Systems and methods for universal tail-based indexing strategies for amplicon sequencing
专利权人:
Arizona Board of Regents on behalf of Northern Arizona University;The Translational Genomics Research Institute
发明人:
Schupp, James M.,Colman, Rebecca,Engelthaler, David,Gillece, John,Hicks, Nathan,Keim, Paul S.
申请号:
AU2014346399
公开号:
AU2014346399A1
申请日:
2014.11.10
申请国别(地区):
AU
年份:
2016
代理人:
摘要:
Some embodiments of the invention include a method of preparing a sample for sequencing that includes receiving a sample and amplifying at least one marker within the sample. In some embodiments, amplification of the first marker may include mixing the sample with a first oligonucleotide that comprises a first universal tail sequence and a second oligonucleotide that comprises a second universal tail sequence. In some aspects of the invention, the first universal tail sequence and the second universal tail sequence are different sequences.
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