The invention relates to a process for the extraction of a protein present in milk, having at least one hydrophobic patch and a negative charge at the natural pH of milk, including the following steps of: a) skimming and defatting the milk, b) transferring the defatted and skimmed fraction containing said protein to a chromatographic support onto which is grafted a ligand that is simultaneously hydrophobic and ionic, under pH conditions allowing said protein to be captured on said support, said pH being higher than 4.6, c) eluting the protein, d) purifying the eluted fraction by removing the milk proteins from said eluted fraction, and e) recovering said protein.本發明係關於一種萃取乳汁蛋白質之方法,於該乳汁之天然pH下此蛋白質具有至少一個斥水嵌片及一負電荷,該方法包括下列步驟:a)撇渣且脫脂該乳汁,b)於允許該蛋白質被捕捉於一層析術撐體之pH條件下(該pH係高於4.6),將含有該蛋白質之經脫脂且經撇渣之選分轉移至一層析術撐體,該撐體其上接枝同時為斥水性及離子性之一配體,c)洗提該蛋白質,d)經由自該經洗提之選分移除該乳汁蛋白質來純化該經洗提選分,以及回收該蛋白質。
