The present invention relates to a composition for inducing hypometabolism of fish, a method for inducing hypometabolism, and a fish transportation method using techniques thereof. More particularly, a method for inducing long-term hypometabolism of fish of the present invention enables the ventilation frequency of fish to decrease by treating the fish with T1AM, which is a hypometabolic-inducing substance and enables the ventilation frequency to be recovered to an original state when T1AM is removed. In addition, although the oxygen consumption rate decreases and the survival time increases as the ventilation frequency decreases, there is no change in the expression profile of chaperone which occurs due to stress, such as long-term oxygen deficiency, and a decrease in ventilation frequency by T1AM exhibits the same effect as a case of decreasing water temperature by 10°C. Therefore, by means of the technique, it is possible to provide a fish transportation method which is stable and economical while preventing deterioration of quality of fish meat.