Disclosed in the present invention are a lipidosome preparation, a preparation method and an application thereof in treatment for related diseases caused by abnormal expression of genes. The lipidosome preparation is characterized by comprising a complementary double-positive lipid, phospholipids and a long-acting lipid. The preparation method comprises: forming pre-prepared empty vesicles after mixing the solution of complementary double-positive lipid, phospholipids and long-acting lipid, and then mixing the pre-prepared empty vesicles with nucleic acid solution to form the nucleic acid lipidosome preparation. The lipidosome preparation provided by the present invention is easy to prepare, capable of transmitting medicines such as nucleic acid in vivo to exert a treatment effect, and capable of being used for treating related diseases caused by abnormal expression of genes.
