This invention address that Pemetrexed preparation include nonaqueous solvents keep stablizing at least dilute after about 48 3 hours, when being stored in 2 °C to 8 °C. It include that nonaqueous solvents is kept stablizing at least about in 24 2 °C to 8 °C of storages present invention is alternatively directed to Pemetrexed preparation.