CHANG, FRANK N.,张 芳男,張 芳男,TUSZYNSKI, GEORGE P.,图辛斯基 乔治 Ｐ,圖辛斯基 喬治 Ｐ,LUO, SOLOMON,罗 志伟,羅 志偉,YANG, JEFF,杨 中璜,楊 中璜
申请号：
TW108116302
公开号：
TW201946660A
申请日:
2019.05.10
申请国别(地区):
TW
年份:
2019
代理人:
摘要：
A method for detecting cells having granules expressing G protein coupled receptor-associated sorting protein 1 (GASP-1) or a fragment thereof is provided. The detection method may comprise: (a) contacting the cells with an effective amount of a binding protein, wherein the binding protein comprises an antigen binding fragment that specifically binds GASP-1； and (b) identifying cells having granules bound to the binding protein. The GASP-1 granules may be in the cytosol or on the surface of the cells. Also provided are methods for producing T-cells comprising a chimeric antigen receptor, anti-GASP-1 antibody or a bi-specific binding protein. Further provided are methods for treating GASP-1-mediated disease or inactivating exosomes, microvesicles or oncosomes.本發明提供一種用於偵測細胞之方法，該等細胞具有表現G蛋白偶合受體相關分選蛋白1 (GASP-1)或其片段之顆粒。偵測方法可包含：(a)使細胞與有效量之結合蛋白接觸，其中結合蛋白包含與GASP-1特異性結合之抗原結合片段；及(b)識別具有結合於結合蛋白之顆粒的細胞。GASP-1顆粒可在細胞溶質中或細胞表面上。亦提供用於產生包含嵌合抗原受體、抗GASP-1抗體或雙特異性結合蛋白之T細胞之方法。亦提供用於治療GASP-1介導之疾病或失活化胞外體、微細小胞或癌小體之方法。
